Background and aim: This study aimed to evaluate the gross leaf macro and micro-morphological charactersof Trees. The foliar samples were collected from three locations (Ujam, Pila, and Ichwa) in Makurdi. Eachspecies was identified and assigned a unique accession number. Foliar macro-morphological features suchas leaflet length, width and area were measured and evaluated. Materials and methods Micro-morphologicalcharacterization involving the fixation and initial preservation of epidermal peels before examination underthe microscope was carried out using standard procedures. The micro-morphological features examinedwere focused on the stomatal and epidermal cell characteristics. Results: The macro-characters showedvariation in the sizes of leaflet length, width and area. Nine (9) accessions were evaluated in this work whichincludes; AZUJ, ANUJ, MAUJ, AZPI, ANPI, MAPI, AZIC, ANIC and MAIC. The accession MAPI (Mangifera indica)had the highest leaflet length, while ANUJ (Anacardium occidentale) recorded the highest value of leafletwidth and area across studied locations. Also, stomatal cell length of Azadirachta indica (AZIC), epidermalcell length of Anacardium occidentale (ANUJ) and epidermal cell width of Mangifera indica (MAPI) displayedremarkable variations and each recorded the highest measured values cross accessions examined. The meanvalues for morphological and anatomical characters evaluated differed significantly at P≤0.05. Conclusion:The comprehensive examination of morphological leaf characters provided insights into variability withintree species. Also, data obtained were valuable and showcased unique adaptation and tolerance mechanismof each species during hash environmental conditions.