Maize is the second most important staple crop in terms of area and production in Nepal. A field survey was carried out to assess the economic analysis of Maize seed production in Bagnashkali Rural Municipality (Palpa district) during month of Oct/Sept for cropping year 2022. Palpa district was selected for the study because of its high contribution to Maize seed production. Semi structured questionnaire was designed to collect data in 182 Maize seed cultivating farmer's household in form of interview using Simple Random Sampling. Descriptive statistics, benefit cost ratio (B/C ratio), Stochastic frontier model, Cobb-Douglas production function were used to analyze the data. The result showed that the average family size of household is 5 and involvement of both male and female in maize seed production and post-harvest management was 48% and 52% respectively. The average rate of quality seed Manakamana-3 was Rs. 57.3/Kg. The average total cost was NRs. 50316.37 and average net return was NRs.62522.5, it showed that average B/C ratio was found to be 1.24/ha. The highest cost was incurred on avg. labor cost during production 61.94 % of average total cost followed by machineries (25.97%), fertilizers cum pesticides (8.05%), seed cost (2.24%) and post-harvest labor cost (1.46%). It was found that 99% of farmers were involved in production of certified maize seed certified at regional laboratory, Bhairahawa. Estimated coefficient of inputs like production labor, postharvest labor, DAP, total area, machine, are positive and show significant result to productivity. The major input for seed production was labor, hence more laborers were required for seed production followed by machinery, fertilizers and seed. The study identified that Maize seed production was profitable and has wide market available. However, low productivity was due to lack of irrigation facilities followed by lack of proper training and extension services.